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Produktbeschreibung
The Drummond Nanoject II Auto Injector is a high performance instrument, which has been specifically designed to perform ultra-delicate nanoliter injection procedures into cells, including oocytes and embryos. Positive displacmenet technology and precision microcapillaries togehter with unique features enhance the ease of use, precise delivery of sample and protection of cells. The automated microprocessor control delivers volumes from 2.3 -69 nl (nano-liter) with highest accuracy and precision even when sample contaminants are present. Cells are protected by the remote controlled, non-rotating plunger that eliminates vibration, minimising the risk of cell ruptures. The narrow tips and delivery volume of the Nanoject II enable injections into specimens other than Xenopus. Quieter and smoother than former models, the higher torque motor further reduces tip movement. A novel collet configuration holds the microcapillary more scurely reducing air infiltration and oil leakage. THE NANOJECT II KIT INCLUDES the following components: - Injector head with lead - Control Box - Power Supply - Capillary glass: 2 vials of 100 pieces per vial: 3,5´´ and 7.0´´ - Replacement O-ring kit with standard collet - Allen key for replacing wire plunger - Needle for backfilling capillaries / micropipettes (30 gauge x 2´´ length) - Universal Adaptor to enable fitting to most micromanipulators Injector Tips: Two lengths of borosilicate glass (N-51-A) capillaries are available for use with Nanoject II - 3.5´´ and 7.0´´ and are ready for pulling to the required tolerance. Once the tips are pulled, they must be backfilled with oil (or other non-compressible fluid) before attachment to the injector. Silicone or mineral oil is frequently used for this purpose. Backfilling is performed using a 30 gauge x 2´´ needle and a syringe. The precise operation of the Nanoject II depends greatly on the use of tips prepared from the specific glass provided. For this reasons, it is recommended that only Drummond microcapillaries are used. ACCESSORIES: The injector head should be mounted on a micromanipulator or stereotaxis unit using the universal adaptor supplied with the kit. The Marzhauser MM33 micromanipulator is a popular, manual model equipped with high precision and stable cross-roller bearings, which works constantly without any noticeable wear even after many years of intensive use. The MM33 also has a number of ergonomic benefits. The control knobs for all three axes are arranged at the same level so that it can be easily operated without looking. In addition, the operational controls are at the back of the unit, allowing several devices to be easily installed side by side. A support base is available for the MM3 micromanipulator and is also compatibel with other brands. For convenient hand-free operation, an optional foot switch can be used to control the Nanoject II remotely. OPERATING SPEED: Nanoject II has two speed options for each operations, allowing the user to fill and inject at half speed and facilitating the use of smaller microcapillaries, as well. Handling of more viscous samples has also been advanced, with no further need for tedious re-calibrations of the viscosity changes. OperationSpeed OptionSpeed Fill SpeedFast46 nl/sec Slow23 nl/sec Injection SpeedFast46 nl/sec Slow23 nl/sec Empty SpeedFast230 nl/sec Slow92 nl/sec RECORDING NANOJECT II INJECTOR: The Recording Nanoject II has been specifically developed to improve microvolume injection techniques in the study of connectivity and function of specific brains nuclei. When used in conjunction with an extracellular amplifier, the integrated system enables small volumes to be injected into targeted areas. - Compatible with most popular sterotactic instruments - Supplied with universal adaptor for easy mounting - Supplements stereotactic co-ordinates in guiding the placement of microvolume injections - Provides direct displacement of neuronal tracer or excitotoxin from the dispenser